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Part I: Arabidopsis Resources
1. Handling Arabidopsis and other Brassicaceae: Growth, Preservation of Seeds, Transformation and Genetic Crosses
Christopher Calhoun, Deborah Crist, Emma Knee, Julie Miller, Eva Nagy, and David Somers
2. Bioinformatic Tools in Arabidopsis Research
G. Alex Mason, Alex Cantó-Pastor, Siobhan M. Brady, and Nicholas J. Provart
Part II: Genetic Techniques in Arabidopsis
3. Analyses of Natural Variation: Field Experiments and Nucleotide Diversity for Your Favorite Gene
Carlos Alonso-Blanco, Belén Méndez-Vigo, and F. Xavier Picó
4. Non-sterile Grafting Methods for Arabidopsis
Nien-Chen Huang, and Tien-Shin Yu
5. CRISPR/Cas9-based Genome Editing Toolbox for Arabidopsis thaliana
Daisuke Miki, Gaurav Zinta, Fangnan Peng, Zhengyan Feng, and Jian-Kang Zhu
6. Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration
César Bernat-Silvestre, Vanessa Vieira, Fernando Aniento, and Ma Jesús Marcote
7. Transient Expression of Fluorescent Fusion Proteins in Arabidopsis Protoplasts
Yonglun Zeng, Changyang Ji, Youshun Lin, and Liwen Jiang
8. Photosynthetic and Heterotrophic Cell Suspension Cultures
Enrico Cortese, Luca Carraretto, Barbara Baldan, and Lorella Navazio
9. Genome-wide Association Studies in Arabidopsis thaliana: Statistical Analysis and Network-Based Augmentation of Signals
Tak Lee and Insuk Lee
Part III: Molecular and Cell biological Techniques in Arabidopsis
10. Visualising and Measuring Single Locus Dynamics in Arabidopsis thaliana
Anis Meschichi and Stefanie Rosa
11. Identification and Quantification of Small RNAs
Di Sun, Zeyang Ma, Jiaying Zhu, and Xiuren Zhang
12. Flow Cytometry and Sorting in Arabidopsis
David W. Galbraith and Guiling Sun
13. Live Imaging of Arabidopsis Leaf and Vegetative Meristem Development
Monica Pia Caggiano, Xiulian Yu, Carolyn Ohno, Pia Sappl, and Marcus G. Heisler
14. Using Genetically Encoded Fluorescent Biosensors for Quantitative in vivo Imaging
Akira Yoshinari, Jacob Moe-Lange, Thomas J. Kleist, Heather N. Cartwright, David A. Quint, David W. Ehrhardt, Wolf B. Frommer, and Masayoshi Nakamura
15. Using Tripartite Split-sfGFP for the Study of Membrane Protein-Protein Interactions
Tzu-Yin Liu
16. High-pressure Freezing and Freeze Substitution for Transmission Electron Microscopy Imaging and Immunogold-labeling
Marisa S. Otegui
17. Atomic Force Microscopy to Study Cell Wall Mechanics in Plants
Mateusz Majda
18. Identification and Characterization of Reproductive Mutations in Arabidopsis
María Flores-Tornero, Stefanie Sprunck, and Thomas Dresselhaus
Part IV: Biochemical and Physiological Techniques in Arabidopsis
19. Metabolomic Analysis of Natural Variation in Arabidopsis
Si Wu, Saleh Alseekh, Yariv Brotman, and Alisdair R. Fernie
20. Untargeted Metabolomics of Arabidopsis Stomatal Immunity
Lisa David, Jianing Kang, and Sixue Chen
21. Biotin-based Proximity Labeling Of Protein Complexes in Planta
Madiha Khan, Rajagopal Subramaniam, and Darrell Desveaux
22. Phosphoproteomic Analysis of Plant Membranes
Lin Xi, Waltr
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