Silac: Methods and Protocols - Hardcover

1. Dynamic SILAC to Determine Protein Turnover in Neurons and Glia

Aline R. Dörrbaum, Erin M. Schuman, and Julian D. Langer

2. SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe

Dorothea Anrather, Silvia Bagelova Polakova, Lubos Cipak, and Juraj Gregan

3. Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Quantitative Proteomics

Monika Kopeckova, Marek Link, and Ivona Pavkova

4. pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles

So-Fong Cam Ngan, Neil E. McCarthy, and Siu Kwan Sze

5. Quantification of Protein Palmitoylation by Cysteine-SILAC

Caiyun Fang, Xiaoqin Zhang, and Haojie Lu

6. Comparative SUMO Proteome Analysis Using Stable Isotopic Labeling by Amino Acids (SILAC)

Georgia Chachami and Sina-Victoria Barysch

7. A Super-SILAC Approach for Profiling Histone Post-Translational Modifications

Roberta Noberini, Elisa Longhi, and Tiziana Bonaldi

8. SILAC-Based Quantitative Phosphoproteomics in Yeast

María Luisa Hernáez and Concha Gil

9. Phosphotyrosine Profiling Using SILAC

Keshava K. Datta, Aditi Chatterjee, and Harsha Gowda

10. Chemoproteomic Profiling of Geranyl Pyrophosphate-Binding Proteins

Rong Cai and Yinsheng Wang

11. Quantitative Analysis of the Endoplasmic Reticulum-Associated Proteins Using ER-Localizable Reactive Molecules

Tomonori Tamura and Itaru Hamachi

12. Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC

Alexis Gonneaud, Claude Asselin, Véronique Giroux, and François-Michel Boisvert

13. Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultured Primary Neurons

Guoan Zhang, Katrin Deinhardt, and Thomas A. Neubert

14. Heavy Methyl SILAC Metabolic Labeling of Human Cell Lines for High-Confidence Identification of R/K-Methylated Peptides by High-Resolution Mass Spectrometry

Enrico Massignani, Marianna Maniaci, and Tiziana Bonaldi

15. SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

Alistair Langlands, Hamze Beati, and H.- Arno J. Müller

16. Super-SILAC Quantitative Proteome Profiling of Zebrafish Larvae

Guillermo Aragoneses-Cazorla, Andres Machuca, M. Pilar Buendia-Nacarino, Daniela S. Anunciação, Estefania Garcia-Calvo, and Jose L. Luque-Garcia